Integrin alpha llb beta 3-Dependent ERK Signaling Is Regulated by Src and Rho Kinases in Both Leu33 and Pro33 Polymorphic Isoforms
http://repository.vnu.edu.vn/handle/VNU_123/29794 Platelet integrin alpha llb beta 3 possesses a Leu/Pro polymorphism at residue 33 (Leu33/HPA-la or Pro33/HPA-1b). The Pro33 isoform has been suggested to exhibit prothrombotic features. alpha llb beta 3-expressing CHO (Chinese hamster ovary) celk. on immobilized fibrinogen show activation of the MAP kinase family member ERK2, with an enhanced ERK2 activity in Pro33 cells compared to Leu33 cells. In our present work, we examined how the Leu/Pro polymorphism modulates the ERK2 activation stimulated by 2 differently triggered outside -in signalings. We either treated the CHO cells with Mn2+ or allowed them to adhere to fibrinogen. Moreover, we studied which signaling cascades are involved in ERK2 activation. In contrast to immobilized fibrinogen, Mn2+ did not significantly increase ERK2 activation. However, Mn2+ had a synergistic effect on ERK2 phosphorylation when combined with immobilized fibrinogen. Pro33 cells a...