Ethanol extracts of Scutellaria baicalensisprotect against lipopolysaccharide-induced acute liver injury in mice
http://repository.vnu.edu.vn/handle/VNU_123/11455
Objective:
To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury.
Methods:
Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week.
Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection).
Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR.
Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting.
Results:
Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues.
Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice.
Conclusions:
Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice
Objective:
To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury.
Methods:
Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week.
Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection).
Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR.
Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting.
Results:
Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues.
Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice.
Conclusions:
Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice
| Title: | Ethanol extracts of Scutellaria baicalensisprotect against lipopolysaccharide-induced acute liver injury in mice |
| Authors: | Nguyen, Thanh Hai Le, Tuan Anh Duong, Thi Ly Huong Nguyen, Huu Tung Vu, Duc Loi Dang, Thu Kim Bui, Thanh Tung |
| Keywords: | Scutellaria baicalensis;Lipopolysaccharide;Liver injury;Cytokine;Nuclear factor-κB |
| Issue Date: | 2015 |
| Publisher: | Elsevier |
| Abstract: | Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods:Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. Results:Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions:Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice |
| URI: | http://repository.vnu.edu.vn/handle/VNU_123/11455 |
| ISSN: | 2221-1691 |
| Appears in Collections: | SMP - Papers / Tham luận HN-HT |
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